TABLE 1:
Yeast strains and plasmids used in this study. Continued
| Strain name | Genotype | Source | Parent | F primera | R primera |
|---|---|---|---|---|---|
| S. cerevisiae strains | |||||
| BY4741 | MATa his3∆1 leu2∆2 met15∆0 ura3∆0 | Giaever et al., 2002 | |||
| BY4742 | MATα his3∆1 leu2∆2 lys2∆0 ura3∆0 | Giaever et al., 2002 | |||
| K1259 | MATa ire1::G418 | Bonilla et al., 2002 | BY4741 | ||
| AK018 | MATa pib2::NAT | This study | BY4741 | Pib2 KO F11 | Pib2 KO R11 |
| AK025 | MATa npr2::HIS3 | This study | BY4741 | Npr2 KO F1 | Npr2 KO R0 |
| AK060 | MATa gtr1::NAT | This study | BY4741 | Gtr1 KO F11 | Gtr1 KO R11 |
| AK061 | MATa gtr2::NAT | This study | BY4741 | Gtr2 KO F11 | Gtr2 KO R11 |
| AK062 | MATa ego1::NAT | This study | BY4741 | Ego1 KO F11 | Ego1 KO R11 |
| AK063 | MATa ego3::NAT | This study | BY4741 | Ego3 KO F11 | Ego3 KO R11 |
| AK109 | MATa iml1::HIS3 | This study | BY4741 | Iml1 KO F11 | Iml1 KO R11 |
| AK069 | MATa npr2::HIS3 gtr1::NAT | This study | AK060 | Npr2 KO F1 | Npr2 KO R0 |
| AK070 | MATa npr2::HIS3 gtr2::NAT | This study | AK061 | Npr2 KO F1 | Npr2 KO R0 |
| AK071 | MATa iml1::HIS3 gtr1::NAT | This study | AK060 | Iml1 KO F11 | Iml1 KO R11 |
| AK072 | MATa iml1::HIS3 gtr2::NAT | This study | AK061 | Iml1 KO F11 | Iml1 KO R11 |
| AK105 | MATα pib2::G418 | This study | BY4742 | Pib2 KO F11 | Pib2 KO R11 |
| AK101 | MATα gtr1::NAT | This Study | BY4742 | Gtr1 KO F11 | Gtr1 KO R11 |
| AK060-b03 | MATa tor1-I1954S gtr1::NAT | This study | AK060 | ||
| AK102 | MATα tor1-I1954S gtr1::NAT | This study | AK105/060-b03 | ||
| AK104 | MATα tor1-I1954S | This study | AK105/060-b03 | ||
| AK106 | MATα tor1-I1954S pib2::G418 | This study | AK105/060-b03 | ||
| AK107 | MATα tor1-I1954S gtr1::NAT pib2::G418 | This study | AK105/060-b03 | ||
| C. albicans strains | |||||
| RBY1182 | a/α leu2/leu2 his1/his1 | Alby et al., 2010 | |||
| DSY797 | cnb1::HIS1/cnb1::LEU2 | Alby et al., 2010 | |||
| KAY483 | cnb1::HIS1/cnb1::LEU2 cmk2∆/cmk2::SAT1 | Alby et al., 2010 | |||
| KAY481 | cmk2::SAT1/cmk2::SAT1 | Alby et al., 2010 | |||
| Plasmids | |||||
| pSM214 | [MET]-GFP AmpR CEN LEU2 | Mehta et al., 2009 | p415MET25 | ||
| pAK009 | [MET]-GFP-Pib2 | This study | pSM214 | Pib2-F-SpeI | Pib2-Stop-XhoI |
| pAK010 | [MET]-GFP-Pib2(1-49) | This study | pSM214 | Pib2-F-SpeI | Pib2+147R |
| pAK011 | [MET]-GFP-Pib2(1-101) | This study | pSM214 | Pib2-F-SpeI | Pib2+303R |
| pAK012 | [MET]-GFP-Pib2(1-162) | This study | pSM214 | Pib2-F-SpeI | PIB2+488R |
| pAK013 | [MET]-GFP-Pib2(1-312) | This study | pSM214 | Pib2-F-SpeI | PIB2+938R |
| pAK014 | [MET]-GFP-Pib2(1-360) | This study | pSM214 | Pib2-F-SpeI | Pib2 +1080R |
| pAK015 | [MET]-GFP-Pib2(1-428) | This study | pSM214 | Pib2-F-SpeI | PIB2+1284R |
| pAK016 | [MET]-GFP-Pib2(1-555) | This study | pSM214 | Pib2-F-SpeI | Pib2 +1665R |
| pAK017 | [MET]-GFP-Pib2(1-620) | This study | pSM214 | Pib2-F-SpeI | Pib2 +1860R |
| pAK018 | [MET]-GFP-Pib2(50-635) | This study | pSM214 | Pib2+148F | Pib2-Stop-XhoI |
| pAK019 | [MET]-GFP-Pib2(102-635) | This study | pSM214 | Pib2+304F | Pib2-Stop-XhoI |
| TABLE 1: Yeast strains and plasmids used in this study. | |||||
| pAK020 | [MET]-GFP-Pib2(165-635) | This study | pSM214 | PIB2+493F | Pib2-Stop-XhoI |
| pAK021 | [MET]-GFP-Pib2(304-635) | This study | pSM214 | PIB2+910F | Pib2-Stop-XhoI |
| pAK022 | [MET]-GFP-Pib2(440-635) | This study | pSM214 | PIB2+1318F | Pib2-Stop-XhoI |
| pAK023 | [MET]-GFP-Pib2(-∆FYVE) | This study | pSM214 | Pib2 1582-FYVEF | Pib2 1326-FYVER |
| pCK294 | Gtr1-i (-S20L) CEN URA3 | Gao and Kaiser, 2006 | |||
| pCK295 | Gtr1-h (-Q65L) CEN URA3 | Gao and Kaiser, 2006 | |||
| pCK296 | Gtr2-h (-S23L) CEN URA3 | Gao and Kaiser, 2006 | |||
| pCK297 | Gtr2-i (-Q66L) CEN URA3 | Gao and Kaiser, 2006 | |||
| pMS034 | Par32-HA CEN URA3 | Huber et al., 2009 | |||
aSequences of forward and reverse PCR primers are available upon request.