Figure 1.

Deletion of ADF and CFL1 Causes Epidermal Thickening and Loss of Tissue Homeostasis

(A) Paraffin-embedded (PE) skin sections from K14CreER^T2 (ADF^+/+ CFL1^+/+), K14CreER^T2/ADF^−/−/CFL1^WT/WT (ADF^−/− CFL1^+/+), K14CreER^T2/ADF^WT/WT/CFL1^fl/fl (ADF^+/+ CFL1^−/−), and K14CreER^T2/ADF^−/−/CFL1^fl/fl (ADF^−/− CFL1^−/−) mice treated with 4-OHT were stained with H&E. The scale bar represents 100 μm.

(B) BrdU staining of PE skin sections from K14CreER^T2 (ADF^+/+ CFL1^+/+)- and K14CreER^T2/ ADF^−/−/CFL1^fl/fl (ADF^−/− CFL1^fl/fl)-labeled mice, treated for 2 or 4 days with 4-OHT (4-OHT × 2 and 4-OHT × 4, respectively). The scale bar represents 100 μm. Quantification is shown (right panel), with box plots displaying the full range of variation (min to max). Greater than or equal to seven fields/mouse were quantified for two mice/treatment/genotype. ^∗∗∗Mann-Whitney p value < 0.0001.

(C and D) Immunofluorescent (IF) staining for E-cadherin of skin sections and keratinocytes isolated from mouse tails. Solid arrows indicate the presence of E-cadherin in cell-cell contacts whereas broken arrows indicate its absence. The scale bars represent 20 μm.

(E) IF staining of skin sections for Keratin 14 or Keratin 10. The scale bar represents 20 μm.

(F) Immunohistochemical (IHC) (upper panels) and IF staining (lower panels) for Keratin 6. The scale bars represent 100 μm for IHC and 20 μm for IF.

(G) IHC of PE skin sections for cleaved caspase 3. The scale bar represents 100 μm.

Nuclei were counterstained with DAPI in IF images. See also Figures S1A, S2, and S7.