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. 2015 Oct 19;36(6):1487–1496. doi: 10.3892/ijmm.2015.2380

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Lack of cytochrome c (Cytc) protein expression in the cytosolic fraction. (A) The Cytc in the cytosolic fraction was analyzed by western blot analysis. α-tubulin was used as an internal control for cytosolic protein. (B) Cytc in the mitochondria was analyzed by western blot analysis. COX IV protein served as an internal control for mitochondrial protein. (C) Quantitative analysis of Cytc protein expression in mitochondria. Data represent the means ± SEM. Three independent experiments were performed. Ad-GFP, adenovirus green fluorescent protein; Ad-Hsp75, adenovirus heat shock protein 75.