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. 2015 Oct 9;36(6):1497–1506. doi: 10.3892/ijmm.2015.2363

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Copyright: © Song et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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miR-21 targets the 3′-UTR of Smad7 mRNA. (A) RT-qPCR of miR-21 expression in HEK-293, C2C12 cells and MEFs. (B) The sequence of miR-21 complementation with Smad7 3′-UTR wild-type and mutant-type reporter. In mice and humans, miR-21 complementation with Smad7 3′-UTR wild-type is shown. (C) The Smad7 3′-UTR wild-type (200 ng) and mutant-type (200 ng) reporter and pMIR-Cont (200 ng) were co-transfected with miR-21 (100 nM) into HEK-293 cells. After 48 h, luciferase activities were measured. The data shown represent the means + SD; ^*p<0.5, ^**p<0.01 and ^***p<0.001 vs. Pro-MIR.