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. 2015 Dec 15;9:479. doi: 10.3389/fncel.2015.00479

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Copyright © 2015 Spagnuolo, Mollica, Maresca, Cavaliere, Cefaliello, Trinchese, Scudiero, Crispino and Cigliano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serum level of inflammation markers. TNF-alpha (open bar) and Haptoglobin (Hpt; black bar) concentrations were measured by ELISA in serum of rats fed high-fat diet (HFD) or standard diet (control diet, CD) for 12 (CD 12, N = 8; HFD 12, N = 6) or 24 (CD 24, N = 7; HFD 24, N = 7) weeks. Each sample was analyzed in triplicate. Data were expressed as ng/ml (TNF-alpha) or mg/ml (Hpt), and reported as mean ± SEM. Significant differences between groups are indicated (^∗, values significantly different from control by Student’s t-test, p < 0.05).