Table 1.
Functional categorization of newly identified proteins from the extended eyespot fraction F2Ae.
| Transcript name (Phytozome) | No. of different peptides | Function and/or homologies | TMDsa |
|---|---|---|---|
| Proteins important for eyespot development/retina related proteins | |||
| Cre02.g105600.t1.3 | 4 | Eyespot assembly protein EYE3, ABC1 kinase family | (+) |
| Cre12.g547300.t1.3 | 3 | Eye Pigment Precursor Transporter (EPP) family protein | + |
| Cre12.g488350.t1.3 | 2 | Retinal pigment epithelial membrane receptor | (+) |
| SOUL heme-binding proteins | |||
| Cre13.g566850.t1.2 | 2 | Similar to SOUL2 | (+) |
| Cre06.g292400.t1.3 | 2 | Similar to SOUL5 | (+) |
| 14-3-3 proteins | |||
| Cre12.g559250.t1.2 | 10 | 14-3-3 protein | - |
| Cre06.g257500.t1.2 | 8 | 14-3-3 protein | - |
| Kinasesb | |||
| Cre17.g705000.t1.2 | 11 | Calcium-dependent protein kinase 1 | (+) |
| Cre09.g407800.t1.3 | 10 | ABC1/COQ8 ser/thr kinase with an AarF (predicted unusual kinase) domain | (+) |
| Cre16.g663200.t1.3 | 10 | Cyclic nucleotide dependent protein kinase | (+) |
| g8097.t1 | 9 | Predicted protein with AarF (predicted unusual protein kinase) domain | (+) |
| g8097.t2c | 5 | Predicted protein with AarF (predicted unusual protein kinase) domain | (+) |
| Cre13.g570350.t1.3; Cre13.g570350.t2.1 | 6 | ABC-1-like kinase with an AarF (predicted unusual protein kinase) domain | + |
| g13907.t1 | 4 | Predicted protein with AarF (predicted unusual protein kinase) domain | (+) |
| Cre06.g307100.t1.3 | 3 | ABC1/COQ8 ser/thr kinase with an AarF domain | (+) |
| Cre16.g657350.t1.2 | 3 | Protein with catalytic domain of serine/threonine protein kinases | (+) |
| Cre03.g168150.t1.2 | 2 | Protein with catalytic domain of tyrosine kinase | (+) |
| Cre13.g571700.t1.3 | 2 | Protein with catalytic domain of serine/threonine protein kinases | (+) |
| g17359.t1 | 2 | Protein with catalytic domain of serine/threonine protein kinases | (+) |
| Phosphatasesb | |||
| Cre06.g292550.t1.2 | 3 | Protein phosphatase 1 | - |
| Cre09.g388750.t1.2 | 3 | Phosphoinositide phosphatase | + |
| Cre06.g257850.t1.2 | 2 | Serine/threonine protein phosphatase | - |
| Cre01.g030200.t1.2 | 2 | Protein phosphatase 2C-like protein | - |
| Calcium-sensing and binding proteins | |||
| Cre11.g468450.t1.2 | 6 | Similar to centrin | - |
| Cre14.g615750.t1.1 | 4 | Protein with EF-hand, calcium binding motif | (+) |
| Cre12.g559450.t1.3 | 4 | Protein with a C2 domain (found in kinases and membrane trafficking proteins) | + |
| Cre03.g150300.t1.2 | 3 | Protein with EF-hand, calcium binding motif | - |
| Cre03.g178150.t1.1 | 2 | Similar to calmodulin | - |
| Cre15.g641250.t1.2 | 2 | Protein with EF-hand, calcium binding motif | - |
| Membrane-associated/structural proteins, proteins with PAP-fibrillin domain | |||
| Cre13.g583550.t1.2 | 11 | VIPP1, Vesicle inducing protein in plastids 1 | - |
| Cre03.g197650.t1.2 | 8 | Protein with PAP-fibrillin domain | (+) |
| Cre12.g502250.t1.2 | 4 | Protein with PAP-fibrillin domain | + |
| Cre12.g492600.t1.2 | 3 | Fasciclin-like protein | + |
| Cre12.g492650.t1.2 | 2 | Fasciclin-like protein | + |
| Cre11.g478850.t1.2 | 2 | Protein with PAP-fibrillin domain | (+) |
| Transporter | |||
| Cre10.g459200.t1.2 | 11 | Plasma membrane-type proton ATPase | + |
| Cre04.g220200.t2.1; Cre04.g220200.t1.2; Cre04.g220200.t3.1 | 11 | K+/H+-efflux antiporter 2 (KEA2, chloroplast inner envelope) | + |
| Cre09.g388850.t1.1 | 4 | Calmodulin binding calcium-transporting ATPase (P-type/plasma membrane) | + |
| Cre03.g164600.t1.2; | 4 | Plasma membrane hydrogen ATPase | + |
| Cre03.g165050.t1.2 | 2 | Plasma-membrane proton-efflux P-type ATPase | + |
atransmembrane domains, predictions done with TMHMM, TMpred, and TopPred (+, TMDs predicted by all three programs; (+), TMDs predicted by two programs; –, TMDs predicted by only one or no program); bKinases and phosphatases that are putative signaling related; cSplice variant of g8097.t1. Function and/or homologies of depicted proteins were determined by NCBI BLASTp.