Table 3.
Methylated proteins of unknown function from the eyespot fractions F2Ae and F1 (Schmidt et al., 2006).
| Transcript name (Phytozome) | Function and/or homologies | Methylated peptide | z | Xcorr | x-times found |
|---|---|---|---|---|---|
| Cre06.g263250.t1.3 | No significant hit in NCBI BLASTp | AAVADATGAASSAAADAKm2a | 2 | 5.71 | 7 |
| AAVADATGAASSAATDAKm2a | 2 | 4.20 | 3 | ||
| Cp genome | ORF1995 unknown protein | MALEDLSKm3WKm3a,b | 2 | 2.71 | 5 |
| SFDITSMTTTLPFYAGWDESLKm2a | 2 | 4.61 | 3 | ||
| –>SFDITSMoTTTLPFYAGWDESLKm2a | 2 | 3.59 | 1 | ||
| Cre01.g000900.t1.2 | Similar to conserved plant/cyanobacterial proteins of unknown functions, contains two DUF1350 domains | LATVAGQLGVSAATAPLEELSRma,b | 2 | 4.03 | 4 |
| FKDDSLDDTNNLVQLLQGSSSVGEVLDLTVRmb | 3 | 4.35 | 1 | ||
| Cp genome | ORF2971 unknown protein | VAMoLAELSLSNLSAKm3LDMITDLLVIIDSVRma | 3 | 3.31 | 1 |
| MoGQRmKmSQITLLEKma | 2 | 2.52 | 1 | ||
| g2947.t1 | No significant hit in NCBI BLASTp | ADGAAATATTAATGVLGAGFAKmADEAAASATTAATGVLGAGFAKm2a | 3 | 3.67 | 1 |
| g14174.t1 | No significant hit in NCBI BLASTp | GLGDVVGMKm3GPAAEINNGRa | 2 | 3.33 | 1 |
| Cre10.g438450.t1.3 | No significant hit in NCBI BLASTp | GWGKm2LPDSGAALPAFLYKmHVLKma | 2 | 3.13 | 1 |
Km, methylated Lys; Km2, dimethylated Lys; Km3, trimethylated Lys (b and y ions of trimethylamin neutral loss events were found in the corresponding spectra indicating that trimethylation rather than acetylation is present); Rm, methylated Arg; Mo, oxidized Met. The peptide that is marked with an arrow shows an identical sequence, but an oxidized Met is present. aPeptide was identified in an analysis for methylated proteins from the extended eyespot fraction F2Ae.bPeptide was identified in an analysis for methylated proteins from the published eyespot proteome (Schmidt et al., 2006). Function and/or homologies of depicted proteins were determined by NCBI BLASTp.