Fig. 5.

Phosphoinositide binding is not required for Rip11 translocation to the cell surface in response to insulin stimulation.

3T3–L1 adipocytes stably expressing GFP–Rip11 (WT or the PI mutant) were serum starved and stimulated with or without insulin before fixation. (A) Shows images of the cells treated in the absence or presence of insulin, as indicated, obtained by confocal microscopy. Scale bar represents 10 μm. (B) The graph represents mean ± SEM of the amount of Rip11 at the cell surface normalised to total Rip11 expression. (N = 3, n = 30 cells). *p < 0.05, t-test.