Fig. 3.

Inhibitor-resistant mutants are more thermolabile than WT virus. (A) Thermolability curves of EV71 WT (filled circle) and inhibitor-resistant EV71 (open square), WT EV71 in the presence of 2 nM NLD (open circle), inhibitor-resistant EV71 in the presence of 0.9 nM GPP3 and 0.1 nM NLD (open triangle) and 2 nM NLD (filled triangle) (B) Thermolability curves of WT CVA16 (filled circle) and inhibitor-resistant CVA16 (open square). All samples were heated at a range of temperatures for 30 min using a thermocycler, prior to titration by TCID₅₀ assay. Samples containing NLD were first diluted to a level at which the inhibitor has no effect before titration. Titrations were carried out in triplicate and error was measured using standard deviation.