Figure 4.

A). Internucleosomal fragmentation of cells treated with auraptene, umbelliprenin, and herniarin for 48 h. After harvesting the cells, isolated DNA was analyzed by agarose gel elctrophoresis. Lane 1, untreated cells; lanes 2, 3, and 4 cells treated with 70, 80, and 200 µM, auraptene, umbelliprenin, and herniarin, respectively. (B). Western blot analysis of Bax protein expression in MCF-7 cells. Lane 1, untreated cells; lanes 2, 3, and 4 cells treated with 70, 80, and 200 µM, auraptene, umbelliprenin, and herniarin, respectively for 48 h. β-actin was used as a loading control