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. 2015 Oct 5;54(48):14559–14563. doi: 10.1002/anie.201506592

Figure 2.

Figure 2

High-yielding incorporation of three of the four ribonucleotides opposite their complementary bases in aqueous condensation buffer with in situ activation. a) Extension of primer by GMP after 2 d at 20 °C; b) consecutive extension of primer by GMP and CMP after 6 d at 20 °C; c) extension of primer by UMP after 5 d at 0 °C. In each case, the reaction scheme is shown next to a MALDI mass spectrum; conditions: 0.8 m EDC, 0.1–0.15 m ethylimidazole, 0.4 m NaCl, 0.2–0.5 m HEPES buffer, and/or 0.08–0.16 m MgCl2. See the Supporting Information for details.