Fig. 6.

Melanoma cell invasion along and across the meningeal linings of the brain. a GFP-fluorescence images of the dorsal and ventral surfaces of the brain after intracerebral injection of D-12 cells. Arrow indicates injection site. b GFP-stained histological brain sections after intracerebral injection of R-18 cells. GFP-expressing tumor deposits are present on the dorsal and ventral surfaces of the cerebrum (upper panel) as well as on the ventral surface of the midbrain (middle panel) and cerebellum (lower panel). The three brain sections were obtained ~1 mm anterior, ~3 mm posterior, and ~6 mm posterior to the injection site. c–d Histological brain sections showing parenchymal invasion of U-25, A-07, and D-12 cells. e Histological brain sections showing D-12 cells invading the cerebellum and brain stem after intracerebral injection