Table 2.

Comparison of methods to regulate FBA

Method	Associated software	Requires	Dynamic	Steady-state	Multistate	Regulatory	Network
		external data	analysis	analysis	support	loops support	inference
RSA + FBA	Yes (FlexFlux)	No	No	Yes	Yes	Yes	No
RFBA	Yes (COBRA and FlexFlux)	No	Yes	No	COBRA: No FlexFlux: Yes	Yes	No
SRFBA	No	No	No	Yes	No	No	No
iFBA	No	No	Yes	No	No	Yes	No
PROM	No	Yes	Yes	No	No	Yes	Yes