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. 2015 Dec 15;14:208. doi: 10.1186/s12943-015-0480-4

Fig. 4.

Fig. 4

UCP2 was identified the target gene of miR-214. a RT-qPCR was performed to analyze the expression of miR-214 relative to RNU6 in 20 pairs of breast cancer tissues. b RT-qPCR was also performed to analyze the expression of UCP2 mRNA in breast cancer tissues. c, d) MCF7 cells were transfected with 100 nM miR-214 mimics, then RT-qPCR was performed to analyze the expression of miR-214 and UCP2 mRNA. **P < 0.01 vs. negative control (NC). e MCF7 cells were transfected with 100 nM miR-214 mimics or inhibitors for 48 h. The expression and location of the Rhodamine-labeled UCP2 were determined by the immunofluorescence assay. Hoechst 33342 was used to stain nuclei. Images were acquired by confocal microscopy. f MCF7 cells were transfected with luciferase constructs and miR-214 mimics. The comparison of luciferase activity of wild-type (WT) and mutant (MUT) UCP2 constructs was performed 36 h after transfection. Data was normalized to renilla activity. **P < 0.01 vs. negative control (NC)