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. 2015 Dec 15;14:208. doi: 10.1186/s12943-015-0480-4

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© Yu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — UCP2 modulated the 4-OHT/FUL-induced apoptosis in breast cancer cells. a-c MCF7 cells were transfected with UCP2 plasmid and empty vector pcDNA3.1 or 200 nM siRNA of UCP2 for 48 h. RT-qPCR and Western blotting were performed to determine the expression of UCP2. The ratio UCP2/β-actin is quantified (**P < 0.01 vs. empty vector control). d The immunofluorescence assay was performed to analyze the expression and location of UCP2 in MCF7 cells. e Annexin V-PI staining assay was performed to determine apoptosis in MCF7 cells. Bar graphs indicated the percentage of apoptotic cells. **P < 0.01 vs. negative control (NC) or empty vector control (pcDNA3.1)