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. 2015 Oct 20;43(22):11031–11046. doi: 10.1093/nar/gkv1073

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Nuclease footprinting with DNA bubble substrate. (A) Sequence of top (G-strand) and bottom (T-strand) of DNA bubble substrate. The topoisomerase I cleavage site is shown by arrow on the G-strand. Nuclease footprint of EcTOP1-G116S on the bubble substrate is followed by 5′ ³²P end-labeling of either the G-strand or T-strand. (B) DNase I footprinting. Lane 1: Control (C), DNA only; Lane 2: Nuclease only; Lanes 3–7: 10, 20, 40, 60, 100 ng ETOP-G116S followed by nuclease; Lane 8: 100 ng of EcTOP1-G116S only. (C) MNase footprinting. Lane 1: Control (C), DNA only; Lane 2: Nuclease only; Lanes 3–6: 10, 20, 40, 60 ng ETOP-G116S followed by nuclease. Nuclease cleavage products are denoted by * symbol.