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. 2015 Nov 3;43(22):10870–10881. doi: 10.1093/nar/gkv1154

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — The effect of consecutive nucleotide additions on DNA association and dissociation by EcoP15I. (A) EcoP15I-DNA dissociation measured in the SPR assay was initiated by consecutive injections of ATP for 1 s (arrows) followed in each case by Buffer R+ without nucleotide. (B) Kinetic constants are shown from fits of the EcoP15I–DNA dissociation profiles following each ATP pulse in panel A to an offset single exponential function. The change in the amplitude as a function of total reaction time appears approximately linear. (C) EcoP15I–DNA dissociation measured in the SPR assay was initiated by consecutive injections of: ATP for 1 s (arrow) followed by Buffer R+; AMP–PNP for 180 s (grey block) followed by Buffer R+; ADP for 180 s (grey block) followed by Buffer R+; and, ATP for 1 s (arrow) followed by Buffer R+. Kinetic rate constants and fractional amplitudes are shown for the single exponential dissociation produced by each ATP pulse.