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. 2015 Nov 17;43(22):10907–10924. doi: 10.1093/nar/gkv1225

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Fluorescence quenching as a reporter of adjacent binding between Alexa647-labelled ssoSSB monomers. (A) Fluorescence spectra of Alexa647-labelled SsoSSB as function of increasing concentrations of a 12-mer dC single-stranded DNA. (B) Percentage of quenching of Alexa647 intensity as a function of ssDNA concentration in a background of 10 mM KCl. Values represent the average of three experiments and are given as mean ± s.e.m. Solid line indicates the non-linear squares fit to Equation S1 as described in the Supplementary section. K_D and Hill coefficient values of 20 ± 1 nM and 1.3 ± 0.2 were obtained, respectively.