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. 2015 Nov 17;43(22):11047–11060. doi: 10.1093/nar/gkv1256

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — SNM1B-S183A-S330F is impaired for the digestion of both native DNA and ICL-containing substrates. (A) Time-course nuclease assay of SNM1B and SNM1B-S183A (0.4 nM) mutant using 3′ radio-labeled (α-³²P-dATP) 21-mer single- and double-stranded DNA (100 nM) quenched at the indicated times. (B) Time-course nuclease assay of SNM1B and SNM1B-S183A (0.4 nM) mutant using 3′ radio-labeled (α-³²P-dATP) 21-mer double-stranded DNA with a centrally-located SJG-136 cross-link (100 nM), quenched at the indicated times. (C) Quantification using ImageJ of the 11nt product band from three repeats of nuclease assay of cross-linked DNA with SNM1B and SNM1B-S183A, error bars show the standard error of the mean.