Figure 3.

The C-terminal domain of MutL and β form a specific complex in solution. (A) E. coli MutL^CTD (eL^C), MutL^CTD* (eL^C*), and β (eβ) were purified and equimolar mixtures of either eL^C-eβ or eL^C*-eβ (lanes marked with an asterisk) were incubated in the absence of reducing agent. Samples withdrawn from the reaction at the indicated time points were resolved on denaturing gels in the absence of β-mercaptoethanol (β-ME). The right panel is a control gel run in the presence of β-ME. (B) The inactive variants of B. subtilis MutL^CTD (bL^CI), MutL^CTD* (bL^CI*), as well as β (bβ) were purified and equimolar mixtures of either bL^CI-bβ or bL^CI*-bβ (lanes marked with an asterisk) were incubated in the absence of reducing agent. Samples withdrawn from the reaction at the indicated time points were resolved on denaturing gels in the absence of β-mercaptoethanol (β-ME).