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Full length MutL and β form a specific complex in solution. E. coli MutL (eL), MutL* (eL*), and β (eβ) were purified and equimolar mixtures of either eL-eβ or eL*-eβ were incubated in the absence of reducing agent. Samples withdrawn from the reaction at the indicated time points were resolved on denaturing gels in the absence of β-mercaptoethanol (β-ME).
