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. 2015 Sep 17;43(22):10746–10759. doi: 10.1093/nar/gkv918

Figure 7.

Figure 7.

β stimulates the nicking activity of MutL and MutLCTD. (A) Full length B. subtilis MutL (bL; 303 nM) was incubated with a 200 base-pair radiolabelled DNA substrate (10 nM) in the absence or presence of equimolar β (bβ). The reaction was also performed in the presence of 2 mM EDTA where indicated. (B) bMutL and bMutL* (152–303 nM) were incubated with 200 base-pair DNA in the absence and presence of equimolar bβ. (C) bL, bL mixed with equimolar bβ, and crosslinked bL-bβ (152–606 nM) were incubated with 200 base-pair DNA. (D) bMutLCTD and bMutLCTD* (1.15–2.3 μM) were incubated with 200 base-pair DNA in the absence and presence of equimolar bβ.