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. 2015 Sep 17;43(22):10760–10781. doi: 10.1093/nar/gkv934

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 10. — Silencing of CITED2 impairs p300 and ERCC1 protein levels, increases DNA damage and reduces cell viability in cancer HeLa cells. (A) Silencing efficacy of CITED2 by shRNA. (B) Inhibition of p300 and ERCC1 protein levels and accumulation of γ-H2AX levels following CITED2 silencing. (C) Quantification of accumulation of γ-H2AX levels following CITED2 silencing as shown in (B). (D) Quantification of reduction of ERCC1 protein levels following CITED2 silencing in (A). (E) Quantification of reduction of p300 protein levels following CITED2 silencing as shown in (A). (F) Inhibition of ERCC1 mRNA levels by CITED2 silencing. (G) Inhibition of p300 mRNA levels following CITED2 silencing. (H) Potentiation of cisplatin induced inhibition of cell viability by CITED2 silencing and rescued by ectopic expression of ERCC1. (I) Inhibition of ERCC1 protein levels following p300 silencing. (J) Quantification of ERCC1 and p300 protein levels following p300 silencing as shown in (I). (K) Potentiation of cisplatin-induced inhibition of cell viability by p300 silencing and rescue by ectopic expression of ERCC1. (L) Reduction of ERCC1, p300 and chromatin-relaxation markers following CITED2 silencing is rescued by ectopic expression of CITED2. HeLa cells receiving the indicated treatments were processed for immunoblotting. Quantification of protein levels is shown in Supplementary Figure S2A–C.