Figure 3.

Ectopic expression of ERCC1 rescues shCITED2-induced DNA damage and cell viability. (A) Overexpression of ERCC1 gene reduced γ-H2AX levels. While ERCC1 expression reduced the DNA damage in both control and cisplatin-treated cells (compare lanes 1–3 and 7–9), ERCC1 expression partly reduced shCITED2 potentiated γ-H2AX levels (compare lanes 5 and 11). (B) Quantification of DNA damage shown in (A). (C) Protection of cisplatin-induced cell growth inhibition by ERCC1 overexpression. (D) Protection of cisplatin-induced and/or shCITED2 sensitized cell death by ERCC1 overexpression.