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. 2015 Sep 17;43(22):10760–10781. doi: 10.1093/nar/gkv934

Figure 4.

Figure 4.

Silencing of CITED2 downregulates cisplatin-induced ERCC1 mRNA levels which were partly suppressed by the p53 inhibitor PFTα. (A) ERCC1 protein instability remained similar in both control and shCITED2-treated cells. Cells were pre-treated with cycloheximide (CHX) for 2 h followed by incubation in CHX-free culture medium for the indicated time. (B) Quantification of ERCC1 protein levels shown in (A). ERCC1 protein level was only minimally affected by shCITED2 as indicated by similar curve slopes. (C) Reduction of ERCC1 mRNA levels by shCITED2. (D) CITED2 mRNA levels remained suppressed by shCITED2. (E) Reduction of cisplatin-induced ERCC1 mRNA levels by shCITED2 is less dramatic in the presence of PFTα. Modulation by PFTα was greater at the low cisplatin concentration used. (F) CITED2 mRNA remained at low levels following shCITED2 expression. mRNA levels of CITED2 appeared to fluctuated in the presence of PFTα. Fold difference of average mRNA levels and P-values are indicated (n = 3).