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. 2015 Sep 17;43(22):10760–10781. doi: 10.1093/nar/gkv934

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — Silencing of p300 reduces chromatin relaxation, H3Ac/p53 complex loading on the ERCC1 promoter and sensitizes cells to cisplatin. (A) Silencing efficacy of CITED2 by shRNA. p300 mRNA levels were determined in untreated or cisplatin-treated HEK293 cells. (B) Reduction of ERCC1 protein levels following silencing of p300. Quantification of p300 protein levels of (A) is shown. (C) Reduction of ERCC1 mRNA levels following p300 silencing. Quantification of p300 mRNA levels of (A) is shown in the right panel. (D) ChIP data indicating reduced binding of p53 to the ERCC1 gene following p300 silencing. Representative PCR product levels are shown on top. The binding level of triplicates was presented as described in Figure 5. (E) Reduced binding of H3K9Ac to ERCC1 gene following p300 silencing. (F) Reduced binding of H3K14Ac to ERCC1 gene following p300 silencing. (G) Reduced binding of H3K9Ac/p53 to ERCC1 gene following p300 silencing. (H) Reduced binding of H3K14Ac/p53 to ERCC1 gene following p300 silencing. (I) Sensitization of HEK293 cells to cisplatin following shp300 expression is rescued by ectopic expression of ERCC1.