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Nrf2 negatively controls the basal expression of Txnip in mouse heart. Heart tissues from adult male WT and KO mice were collected. A, Txnip mRNA expression. Total RNA was analyzed for Txnip mRNA expression by real-time PCR (n = 4 for each genotype). B, TXNIP protein expression. Heart homogenate pooled from four mice was examined for TXNIP protein levels by immunoblotting.
