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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Mol Pharmacol. 2012 Aug 6;82(5):887–897. doi: 10.1124/mol.112.081133

Fig. 5.

Fig. 5

Nrf2 inhibits the basal and induced transcription by Txnip promoter. A, induction of Txnip promoter-driven luciferase expression. The Txnip promoter sequence from −1 to −2000 was sub-cloned into the pGL3-basic/Luc vector to generate the promoter-reporter plasmid pTxnip-P. H9C2 cells were transfected with the plasmid, followed by treatment with glucose. Luciferase activity was measured. B, suppression of Txnip promoter transcription by overexpressing Nrf2. H9C2 cells were cotransfected with pTxnip-P and pCMV-Nrf2, followed by treatment with glucose. C, increase in Tx-nip promoter transcription by knocking down Nrf2. H9C2 cells were cotransfected with pTxnip-P and Nrf2 siRNA, followed by treatment with glucose. Luciferase activities were measured and are expressed as means and S.D. from 3 samples. *, p < 0.05; **, p < 0.01.