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Nrf2 regulates Trx activity through TXNIP. H9C2 cells were transfected with Txnip siRNA or Nrf2 siRNA followed by treatment with glucose. Cell lysate was prepared, and Trx activity was measured to show suppression of Trx by high glucose (A) or regulation of Trx activity by TXNIP and Nrf2 (B). Data represent means and STD from three samples. *, p < 0.05; **, p < 0.01; and ***, p < 0.001.
