TABLE 3.
Antigen | Immunogen | Source, species, type, catalog number |
Dilution | Reference, RRID |
---|---|---|---|---|
Green fluorescent protein (GFP) |
GFP isolated directly from Aequorea victoria |
Life Technologies, A-11120 Mouse monoclonal (IgG2a) |
1:400 | (Ting et al., 2014) RRID: AB_10055152 |
Green fluorescent protein (GFP) |
full-length GFP | Life Technologies, G10362 Rabbit monoclonal |
1:400 | (Ting et al., 2014) RRID: AB_10565179 |
Green fluorescent protein (GFP) |
recombinant full length GFP protein |
Abcam, ab13970 Chicken polyclonal |
1:1000 | (Ting et al., 2014) RRID: AB_300798 |
HA | synthetic peptide (residues 76- 111 of X47 hemaglutinin 1) |
Roche, 11867423001 Rat monoclonal (IgG1) |
1:400 | (Karuppudurai et al., 2014) RRID: AB_390918 |
Horseradish peroxidase(HRP) |
purified peroxidase from horseradish |
Abcam, ab10183 Mouse monoclonal (IgG2b) |
1:2000 | (Karuppudurai et al., 2014) RRID: AB_296913 |
FasIII | nerve cord and primary neuronal membrane lysate (10- 13 hr development) |
DSHB, Iowa City, IA Mouse monoclonal (IgG2a) |
1:50 | (Snow et al., 1989) RRID: AB_528238 |
Connectin |
Drosophila Connectin protein expressed in pGEX vector |
DSHB, Iowa City, IA Mouse monoclonal (IgG1) |
1:50 | (Meadows et al., 1994) RRID: AB_10660830 |