Figure 3.

Reaction of F₃Y^•-β2, wt-α2, CDP, and ATP at 25 °C monitored by (A) RFQ-EPR spectroscopy and (B) the RCQ method. All data points represent the averages of two independent trials. Data were fit to a (A) two- or (B) three-phase model with the rate constants shown in Table 1. (B) The inset shows dCDP formation during the first 2 s of the reaction. The rate constants measured for Y₃₅₆^• formation and disappearance correlate with the fitted rate constants for dCDP formation in the first two phases.