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Reaction of F3Y•-β2, wt-α2, CDP, and ATP at 5 °C monitored by (A) RFQ-EPR spectroscopy and (B) the RCQ method. All data points represent the averages of two independent trials. Black lines represent biexponential fits to the data with the rate constants given in Table 1. (B) The inset shows dCDP formation during the first 5 s of the reaction. The rate constants measured for Y356• formation and disappearance are identical to the rate constants measured for dCDP formation.
