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. 2015 Nov 23;112(49):15101–15106. doi: 10.1073/pnas.1514028112

Fig. 3.

Fig. 3.

PNPase motion along RNA. (A) Twenty-two representative single-molecule records of PNPase advancing on 155-bp DNA–RNA hybrid constructs. Enzymes traversing RNA with 70% A+U content over the first 26 nt (blue traces, n = 11) moved an average distance of 23 ± 1 nt (mean ± SE). Enzymes traversing RNA with 70% A+U content over the first 50 nt (AU50; red traces, n = 11) moved a longer average distance of 62 ± 3 nt (mean ± SE). Records were smoothed with a 100-point boxcar window (t = 50 ms). (B) Periodogram analysis of records from the AU50 construct (n = 27) yielded a peak centered at 0.87 ± 0.14 nm−1 (mean ± SD), corresponding to a step size of 6.6 ± 1.1 nt. (C) Periodogram analysis of records of the unmodified 155-bp construct (n = 25; data from the dsRNA and DNA–RNA hybrid are combined) yielded a peak centered at 0.94 ± 0.22 nm−1 (6.1 ± 1.4 nt; mean ± SD).