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Golgi fragmentation in COS-7 cells following depolymerization of microtubules is mediated by recycling of Golgi enzymes through the ER. Images of COS-7 cells coexpressing Man II-FRB-Venus and Ii-FKBP12-Cer following treatment with 100 μg/mL cycloheximide + 5 μg/mL nocodazole ± 250 nM rapamycin for 4 h. (Scale bar: 10 μm.)
