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. 2015 Nov 23;112(49):15136–15141. doi: 10.1073/pnas.1520997112

Fig. 5.

Fig. 5.

Irf5 deletion abrogates vascular destabilization and EndoMT induced by BLM. (A, Left) Evaluation for the extravasation of Evans blue dye injected into the caudal vein of BLM-treated mice. (Right) Quantification of Evans blue dye extravasation by formamide extraction (n = 6). (B) Immunohistochemistry for α-SMA in the skin of PBS- or BLM-treated mice. (Scale bars, 100 µm.) (C) Immunofluorescence staining for FSP1 (green), VE-cadherin (red), and DAPI (blue) in skin samples from each group. Arrows represent FSP1/VE-cadherin double-positive cells. (Scale bar, 100 µm.) (n = 4). (D, Upper) ChIP assay with anti-IRF5 antibody in human dermal microvascular endothelial cells with or without HMGB1 stimulation (10 μg/mL for 24 h) and in Tlr4+/+ and Tlr4−/− murine dermal microvascular endothelial cells. (Lower) Quantification by qRT-PCR (n = 5). *P < 0.05 by two-tailed unpaired t-test.