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. 2015 Nov 23;112(49):15136–15141. doi: 10.1073/pnas.1520997112

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Fig. S1. — (A) Collagen contents measured by hydroxyproline assay with skin samples (n = 5). (B) mRNA levels of the Col1a1, Col1a2, and Mmp13 genes determined by qRT-PCR in WT and Irf5^−/− murine dermal fibroblasts (n = 9). (C) ChIP assay carried out with murine dermal fibroblasts and anti-IFR5 antibody (n = 4). (D) mRNA levels of fibrillogenesis-related genes determined by qRT-PCR in WT and Irf5^−/− murine dermal fibroblasts (n = 9). (E) mRNA levels of collagen metabolism- and fibrillogenesis-related genes determined by qRT-PCR in the skin of WT and Irf5^−/− mice (n = 5). *P < 0.05 by two-tailed unpaired t test.