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. 2015 Nov 23;112(49):E6780–E6789. doi: 10.1073/pnas.1512968112

Fig. 2.

Fig. 2.

Skeletal muscle-specific KLF15 overexpression is sufficient to drive an ergogenic metabolic program in vivo. (A) Schematic of transgenic construct. (B) qRT-PCR from indicated tissues demonstrating skeletal muscle-specific KLF15 overexpression in MTg mice versus control (littermate non-Tg mice; n = 5). (C) Representative histology from quadriceps (WGA staining) and (D) quantification of myocyte cross-sectional area (n = 3 independent mice per group). (Scale bar, 50 µm.) (E) Treadmill exercise (n = 6), (F) wire-hang assay (n = 7–10), and (G) metabolic exercise test (n = 7–10) in MTg and control mice. (H) Heat map of all genes induced in MTg versus non-Tg muscle (Left panel; n = 4). Heat map for the same genes in WT versus KLF15 KO muscle (Right panel, WT levels colored as non-Tg in Left panel) illustrates the reversal in expression versus control in KLF15 KO relative to MTg. (I) GSEA of genes regulated by the GC–KLF15 axis in MTg versus non-Tg muscle. (J) Functional annotation (DAVID) of genes regulated by KLF15 overexpression (*P < 0.05 for indicated comparisons). Data are shown as mean ± SEM.