Fig. S4.

NCR178 is highly expressed in the interzone II-III, and the GFP fusion protein localizes to the peribacteroid space. (A) Spatial expression pattern of NCR178. I, zone I; IId, zone II distal; IIp, zone II proximal; IZ, interzone II-III; III, zone III. (B) NCR178 promoter GUS activity. (Scale bar: 100 µm.) (C) Complementation of dnf4 by C-terminal GFP fusion constructs. NCR211-GFP transformed nodules recover the wild type-like nodule morphology, whereas NCR178-GFP transformed nodules do not. The transgenic nodules were selected by DsRED1 fluorescence from hairy root-transformed roots. NT, nontransgenic root. (Scale bar: 1 mm.) (D) Localization of NCR178-GFP translational fusion in the peribacteroid space. (Scale bar: 10 µm.)