Fig. S1.

Mutations in G₁/S regulatory genes are enriched in KBM-7 cells that survive MK-1775 treatment. (A) KBM-7, MDA-MB-231, and SKOV3 cells were treated with increasing MK-1775 concentrations and viability was assessed 72 h after treatment using methyl thiazol tetrazolium (MTT). Averages and error bars of at least three replicates are shown. (B) KBM-7 cells were treated with MK-1775 (300 nM) for indicated time points and analyzed by Western blotting for indicated proteins. (C) KBM-7 cells were treated with MK-1775 (300 nM) and cell cycle profiles were determined at indicated time points by flow cytometric analysis after staining with propidium iodide. Plotted are the average percentages and SDs of sub-G₁ cells from three experiments. (D) Schematic representation of the genetic screen with the Wee1 inhibitor MK-1775 in KBM-7 cells.