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. 2015 Sep 23;112(49):15244–15249. doi: 10.1073/pnas.1417797112

Fig. 4.

Fig. 4.

Purified HrrP cleaves synthetic NCR peptides. (A) Electrostatic map of the structural model of HrrP derived using I-TASSER online (closest threading model was PDB ID code 1bccA). The outer white dotted line indicates the catalytic chamber, and the inner white dotted line indicates the Zn2+ coordinating residues. (B) Tris-Tricine SDS/PAGE gels showing the time course of NCR079 and NCR129 peptide cleavage by purified HrrP or HrrP (E62A). (C) HPLC analysis of NCR079 cleavage over time (0, 5, 10, and 30 min) by HrrP.