Skip to main content
. 2015 Sep 23;112(49):15244–15249. doi: 10.1073/pnas.1417797112

Fig. 5.

Fig. 5.

The effects of hrrP on bacterial fitness, senescence, and bacteroid morphology in M. truncatula nodules. (A) Bacterial fitness for the strains B800 pHR− (C307) and B800 pHR− with a chromosomal copy of hrrP (PP317), as measured by cfus released from nodules 28 dpi for M. truncatula accessions A20 and A17 (n = 40 nodules per condition). *P < 0.05. (B) qRT-PCR analysis of the fold change in expression of the senescence markers MtCP6 and MtVPE for A20 and A17 nodules induced with B800 pHR− with a chromosomal copy of hrrP (PP317) or B800 pHR− ΔnifH (PP411) compared with B800 pHR− (C307). Error bars in A and B indicate SEM. (C–F) Spatial series of transmission electron microscopy images starting at the middle zone and ending at the root-proximal zone of a single M. truncatula A20 nodule induced with B800 pHR− with a chromosomal copy of hrrP (PP317). The nodule was harvested at 28 dpi. (Scale bars: 1 μm.)