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. 2015 Sep 23;112(49):15244–15249. doi: 10.1073/pnas.1417797112

Table S4.

Plasmids used in this study

Plasmid Relevant features Source/reference
pJG476 pJG194 (25) carrying the acdS upstream region; KmR (12)
pJG542 lacI-Ptac expression vector This study
pPP047 Plasmid carrying a Himar1-based transposon system with T7 promotors oriented toward the chromosome; KmR (26)
pPP101 pJQ200 (27) derivative carrying the acdS upstream region but lacking the sacB gene; GmR This study
pPP105 pJG194 (25) carrying an hrrP internal fragment; KmR This study
pPP119 pJG542 carrying hrrP with a C-terminal His6X-tag This study
pPP123 pJG110 (mini-Tn5) (25) carrying hrrP with a E62A mutation with native promoter; ApR, KmR This study
pPP124 pJG110 (mini-Tn5) (25) carrying hrrP with native promoter; ApR, KmR This study
pPP132 pJG194 (25) carrying an hrrP promoter (474 bp)-GUS fusion; KmR This study
pPP147 pRF771 (28) carry hrrP driven by the trp promoter; TcR This study
pPP156 pJG194 (25) carrying an nifH promoter (523 bp)-GUS fusion; KmR This study
pPP157 pJG194 (25) carrying an flaB promoter (124 bp)-GUS fusion; KmR This study
pPP169 pJG392 (25) carrying an nifH internal fragment; TcR This study
pPP196 pJG542 carrying hrrP with a E62A mutation and a C-terminal His6X-tag This study
pPP255 pJG194 (25) carrying the Ptrp promoter from pRF771 (26)-GUS fusion and a rhaK locus integration sequence; KmR This study