Fig. 4.
Expression of m1C3 and m2A10 transcripts in AsMCRkh2 transgenic females. RT-PCR was used to detect m1C3 (AgCPA-m1C3) and m2A10 (AsVg1-m2A10) transcripts in RNA isolated from homogenates of dissected midguts and the remaining carcasses of mixed heterozygous and homozygous DsRed+ G3 females at 0 (non–blood-fed), 4, 12, 24, and 48 h post blood meal. Male transgenic mosquitoes were used as negative controls. The An. stephensi S26 ribosomal protein transcript was amplified from all samples as a loading control.