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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: J Biomol Screen. 2013 Jul 2;19(1):100–107. doi: 10.1177/1087057113494405

Figure 2.

Figure 2

Experimental procedure flowcharts. (A) Generation of pseudotyped virions. 293T producer cells are cotransfected with plasmid-containing virus envelope protein gene (pEnvGP) and plasmid-containing human immunodeficiency virus (HIV) genome with the luciferase reporter gene but lacking the HIV envelope protein gene (pHIVΔEnv-luc). Forty-eight hours later, pseudovirions in the supernatant are collected for infection assay. (B) Virus infection assay. Pseudovirions and compounds are applied to A549 target cells, and infection is determined 48 h later by luciferase activity assay.