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. 2015 Dec 15;10(12):e0145016. doi: 10.1371/journal.pone.0145016

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© 2015 Balvan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Real-time monitoring of relative cell impedance (showed as a cell index) using the RTCA system. (B) MTT-assessed response to 6h plumbagin treatment. (C) MTT-assessed response to 24h plumbagin treatment. (D) IC50 values according to RTCA and MTT and length of treatment. (E) Time-dependent changes in the quantity of large cells with intact nuclei (SYTO 16++/FSC+) and intact cells (AnnexinV-/PI-) assessed by flow-cytometry. (F) Numbers of large healthy cells depicted as SYTO 16++ /FSC+ (red) cluster at flow-cytometric dot plot at 6h time-point; Forward-scattered light (FSC) is proportional to cell-surface area or size. (G) Granularity of large healthy cells depicted as SYTO 16++ /SSC+ (red) cluster at flow-cytometric dot plot at 6h time-point; Side-scattered light (SSC) is proportional to cell granularity or internal complexity. (H) Morphology of PC-3 cells after 6h plumbagin treatment, 20x magnification, phase contrast microscopy. (I) Numbers of large healthy cells depicted as SYTO 16++/FSC+ (red) cluster at flow-cytometric dot plot at 24h time-point; Forward-scattered light (FSC) is proportional to cell-surface area or size. (J) Granularity of large healthy cells depicted as SYTO 16++/SSC+ (red) cluster at flow-cytometric dot plot at 24h time-point; Side-scattered light (SSC) is proportional to cell granularity or internal complexity. (K) Morphology of PC-3 cells after 24h plumbagin treatment; giant PC-3 cells with polyploid giant cancer cell (PGCCs)-like morphology are highlighted by arrows. 20x magnification, phase contrast microscopy.