Skip to main content
. 2016 Jan 1;6(1):14–27. doi: 10.7150/thno.13515

Figure 6.

Figure 6

Physical characterization and in vivo behavior of siRNA and Pt(IV)-OC co-loaded PEDA micelleplexes: (a&b) Size distribution of (a) PEDA/Pt(IV)-OC micelles and (b) PEDA/Pt(IV)-OC/siRNA micelleplexes; (c) TEM image of PEDA/Pt(IV)-OC micelles, and PEDA/Pt(IV)-OC/siRNA micelleplexes examined at pH = 7.4 or 6.0 (scale bar = 100 nm); (d) In vitro cytotoxicity, and (e) pharmacokinetics of PEDA/Pt(IV)-OC/siRNA micelleplexes; (f) Tissue distribution of Pt(IV)-OC prodrug when delivered with PEDA micelleplexes; (g) In vivo and (h) ex-vivo distribution of siRNA-loaded micelleplex in 4T1 orthotopic tumor-bearing mice (H: heart; L: liver; S: spleen; Lu: lung; T: tumor; the black arrows in Figure g indicated the location of 4T1 tumors); (i) CLSM examination determined intratumoral distribution of siRNA-loaded micelleplexes 2 h post tail vein injection (scale bar = 50 μm). The PEDA micelles and siRNA were labeled with Ce6 and FAM, respectively.