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. 2016 Jan 1;6(1):65–77. doi: 10.7150/thno.12350

Figure 5.

Figure 5

CREG1 is overexpressed in cell harboring KRAS mutations. (A) Total protein expression profile of CLPTM1, TMEM106B, and CREG1 in several NSCLC cell lines with wild-type-KRAS (WT) and varying mutations in KRAS. (B) siRNA targeted knockdown of KRAS reduced expression of CREG1 in KRAS mutant NSCLC cell lines. β-actin served as a loading control. (C) KRAS and CREG1 mRNA levels in H358, H441, and H460 cell lines transfected with KRAS siRNA or control siRNA relative to GAPDH mRNA expression. Error bars represent mean s.d. and **p<0.01. (D) CREG1 expression in lung tumor lysates from patients displaying wild-type (WT) or mutant KRAS.