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. 2016 Jan 1;7(1):42–49. doi: 10.7150/jca.12923

Figure 1.

Figure 1

GM-CSF (skewed towards M1) and M-CSF (skewed towards M2) differentiated macrophages expressed different surface protein distribution in flow cytometry. Comparing GM-CSF and M-CSF macrophages proportion of cells positive to M2 markers CD209, CD163 and CD14 was significantly higher in M-CSF differentiated macrophages but M2 marker CD206 was less positive and also M1 marker CD80 was higher (#p<0.05). This shows a partly incomplete polarization with these commonly used stimulants. MiaPaCa-2 cells increased only the CD16 positivity of GM-CSF macrophages but had no significant effect on M-CSF differentiated macrophages. IL6 induced no significant changes to the surface expression of GM-CSF differentiated macrophages. Adding IL4 and LPS to M-CSF differentiated macrophages increased the proportion of cells positive to M2 markers CD209 and CD206 and to M1 marker CD16 but M2 markers CD163 and CD14 decreased (*p<0.05). Error bars show the standard error of mean.