Skip to main content
. 2016 Jan 1;7(1):50–59. doi: 10.7150/jca.13207

Figure 3.

Figure 3

Figure 3

Figure 3

Immunocytochemistry of uMtCK and HKII in prostate cancer cells. (A) Mitochondria and uMtCK were visualized by immunocytochemistry using MitoTracker Red (middle panel) and uMtCK-specific antibody (left panel), respectively, in the indicated cell lines. (B) Mitochondria and HKII were visualized by immunocytochemistry using MitoTracker Red (middle panels) and HKII-specific antibody (left panel), respectively, in the indicated cell lines. Scale bar = 10 µm. (C) Subcellular localization of uMtCK was examined in prostate cancer cell lysates that had been separated into total, post nuclear supernatant (PNS), and mitochondrial fractions. The indicated proteins were detected by immunoblotting. The expression levels of p32 and VDAC were also detected as markers for mitochondria, whereas B23 (nucleophosmin) was used as a marker for nuclear and cytosolic localization.