Table 1.

Restriction enzymes frequently used in the construction of GWSS libraries.

Enzyme	Recognition Sequence	Dam Blocked?	Dcm Blocked?	CpG blocked?
AluI [11,27]	AG/CT	No	No	No
ApeKI [23,28,34,37,38]	G/CWGC	No	No	Yes
AvaII [25]	G/GWCC	No	Yes	Yes
BsaXI [31]	(9/12)ACNNNNNCTCC(10/7)	No	No	No
DraI [11,20]	TTT/AAA	No	No	No
EcoRI [22,26,33]	G/AATTC	No	No	Yes
EcoRV [11]	GAT/ATC	No	No	Yes
EcoT22I [37]	ATGCA/T	?	?	?
HaeIII [11,20,30]	GG/CC	No	No	No
HincII [11]	GTY/RAC	No	No	Yes
HindIII [29]	A/AGCTT	No	No	No
HpaII [21]	C/CGG	No	No	Yes
MluCI [26]	/AATT	No	No	No
MlyI [11]	GAGTC(N)5/	No	No	No
MseI [21,33]	T/TAA	No	No	No
MspI [24,26,29,40]	C/CGG	No	No	No
NlaIII [26]	CATG/	No	No	No
PstI [10,24,37,38,40]	CTGCA/G	No	No	No
RsaI [11]	GT/AC	No	No	Yes
SbfI [10,12,26]	CCTGCA/GG	No	No	No
SphI [26]	GCATG/C	No	No	No
StuI [11]	AGG/CCT	No	Yes	No
TaqI [33]	T/CGA	Yes	No	No

Source: Reprinted from www.neb.com (2015) with permission from New England Biolabs.